Journal: Frontiers in Cell and Developmental Biology

Article Title: CD44v9 Induces Stem Cell-Like Phenotypes in Human Cholangiocarcinoma

doi: 10.3389/fcell.2020.00417

Figure Lengend Snippet: Cell proliferation study of CD44v9 knockdown condition in CCA cells. (A) KKU213 cells were treated with siRNA, and cell growth was evaluated through MTT assay. (B) Diagram and percentage of cell apoptosis. Data show four cell populations of viable cells (lower left quadrant), early apoptotic cells (lower right quadrant), necrotic cells (upper left quadrant), and late apoptotic cells (upper right quadrant). Y -axis is labeled for viability (dead cell marker; 7-AAD) and X -axis is labeled for cell apoptosis (phosphatidylserine-binding protein; Annexin V). Bar chart presents the percentage of total apoptotic cells (early and late apoptotic cells). (C) Diagram and percentage of cell cycle. Histogram shows the number of cells ( Y -axis) and DNA content ( X -axis) with G0/G1 phase (tallest peak), G2/M phase (rightmost peak) and S phase (between G0/G1 and G2/M phases). The values are the mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001 vs. negative control siRNA cells.

Article Snippet: Cells were treated with various concentrations of fluorouracil (5-FU) (Wako) at 37°C, 5% CO 2 for 24 h. Cell viability was evaluated by adding 10 μL of 5 mg/mL MTT solution (Merck Millipore) and incubated at 37°C for 4 h. After removed cell solution and added DMSO, the reaction was measured at a wavelength of 570 nm.

Techniques: Knockdown, MTT Assay, Labeling, Marker, Binding Assay, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells

doi: 10.1155/2017/7283241

Figure Lengend Snippet: Effects of the medicated serum of WMW on cell proliferation in palmitate-treated HepG2 cells. HepG2 cells were treated with 0.25 mM palmitate for 24 h, and normal serum without WMW (N) or medicated serum containing low (L), middle (M), or high (H) dose of WMW was then added for 24 h. The number of viable cells was examined by the MTT assay.

Article Snippet: After treatment with 0.25 mM palmitate and medicated serum of WMW, cell viability was measured using the MTT reagent (Solarbio, Beijing, China) dissolved in phosphate-buffered saline (PBS, 5 mg/mL).

Techniques: MTT Assay